Cedric Raine

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This investigator is a neuropathologist engaged in studies pertaining to the pathogenesis and neuroimmunology of multiple sclerosis and its animal models. Trained in the U.K., Dr. Raine obtained a PhD in Medicine in 1967, a D.Sc. In Medicine in 1975 and was made a Fellow of the Royal College of Pathologists in 1988. He runs a team of faculty and fellows in a 2500 sq. ft. laboratory located in the Forchheimer Building (1st floor). His research is funded by grants from the NIH, the NMSS and industry. The majority of his work is targeted towards the molecular and immunologic analysis of the MS plaque and the testing of therapeutic strategies in the animal model for MS, experimental autoimmune encephalomyelitis (EAE).

Ongoing projects on multiple sclerosis nervous tissue involves the analysis of cytokine and adhesion molecule profiles, the involvement of tumor necrosis factor ligand and receptor families in MS lesions, cell death pathways, oligodendrocyte responses, remyelination, T cell receptor analysis, heat shock proteins and the re-expression of immature myelin genes during repair of the MS lesion.

Animal experimentation includes the treatment of chronic relapsing EAE in mice with reagents that block pro-inflammatory cytokines, with neurotrophic growth factors, and with strategies that promote a cytokine switch from a pro-inflammatory to a regulatory-type (Th1 (Th2). In the furtherance of these studies, immunologic approaches, immunocytochemistry, morphology, in situ hybridization, FACS analysis, ELISA, RT-PCR, Western blotting and light and electron microscopy are applied. In recent years, numerous Neurology and Pathology residents from the USA and abroad have spent research electives and/or postdoctoral fellowships in this lab and have then moved on to careers both in clinical and research disease-related Neuroscience.

 

Genain, C.P., Cannella, B., Hauser, S.L. and Raine, C.S.: Identification of autoantibodies associated with myelin damage in multiple sclerosis. Nature Med. 5:170-175, 1999.

Pitt, D., Werner, P. and Raine, C.S.: Glutamate excitotoxicity in a model of multiple sclerosis. Nature Med. 6:67-70, 2000.

Selmaj, K., Kowal, C., Walczak, A., Nowicka, J. and Raine, C.S.: Naked DNA vaccination differentially modulates autoimmune responses in experimental autoimmune encephalomyelitis. J. Neuroimmunol. 111:34-44, 2000.

Werner, P., Pitt, D. and Raine, C.S.: Multiple Sclerosis: Altered glutamate homeostasis in lesions correlates with oligodendrocyte and axonal damage. Ann. Neurol. 50:169-180, 2001.

Sun, D., Whitaker, J.N., Huang, Z., Liu, D., Coleclough, C., Wekerle, H. and Raine, C.S.: Myelin antigen-specific CD8+ T cells are encephalitogenic and produce severe disease in C57BL/6 mice. J. Immunol. 166:7579-7587, 2001.

John, G.R., Shankar, S.L., Shafit-Zagardo, B., Massimi, A., Lee, S.C., Raine, C.S. and Brosnan, C.F.: Multiple sclerosis: re-expression of a developmental pathway that restricts remyelination. Nature Med. 8:1115-1121, 2002.

Mokhtarian, F., Roman, C., Huan, C-M. and Raine, C.S.: Semliki Forest Virus-induced demyelination and remyelination - a prominent role for B cells and antibodies. J. Neuroimmunol. 137:30-43, 2003.

Gaupp, S., Pitt, D., Kuziel, W.A., Cannella, B. and Raine, C.S.: Experimental autoimmune encephalomyelitis (EAE) in CCR2-/- mice: susceptibility in multiple strains. Amer. J. Path. 162:139-150, 2003.

Cannella, B., Gaupp, S., Tilton, R. and Raine, C.S.: Differential efficacy of a synthetic antagonist of VLA-4 during the course of chronic relapsing experimental autoimmune encephalomyelitis. J. Neurosci. Res. 71:407-416, 2003.

 

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